Part:BBa_K2760019

Characterization construct for SGP1-NSP4

This construct is meant to be used in characterizations of the signal peptide SP1-Nsp4, it should be excreted into the media by the Sec-dependent (Sec) secretion pathway, the general secretion E. coli route. When this signal peptides are linked to the N-terminus of recombinant proteins, they are critical for translocation and secretion of proteins [1].

This sequence has a T7 promoter (BBa_I712074), it contains a specific medium strenght T7 RBS for E. coli, the signal peptide SP1-Nsp4, a compatible codon optimized for both E.coli and L. rhamnosus iLOV sequence (BBa_K2760024) as the fluorescent reporter protein with a His-Tag (6 histidines) attached to it; finally an double loop terminator (BBa_B0015).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 106
  Illegal AgeI site found at 382
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 314